hello, yes I ahve my son had left his eppi in the car and it was bellow 0 out side and it was in the car for two days, I had just filled the Rx and never had a chance to get it from the car untill two days latter and when I did get it I used it like maybe four days latter on my son and it worked with no problem. It nevered happened again after that but it did still work. I hope this helped you!