I was just tested positive for Herpes Simplex 2. My question is how soon after sexual intercourse can blood work determine if I had been affected? I have had unprotected sex with my ex boyfriend on several occassions and with one other man just recently (a month). My blood results came back positive, can I have gotten the virus with the man I was recently with?

You probably got it from your ex. I've read it takes 3 to 6 months for blood test to test positive. It takes that long for antibodies to build up.