When I had my nuclear stress test, it was two injections (actually, they put an IV in tha back of my left hand to use throughout the test).
Once the IV was in, there is an injection of a radioactive tracer (most common I think are Technetium Tc99m Sestamibi = tradenames Cardiolite or Myoview, or Thallium-201) - this is a mild gamma emmittor (total radiation dose of the test should be in the same ballpark range as a chest X-Ray). You go off and sit for a bit, to allow the tracer to circulate and accumulate in the heart muscle cells. Then you have pictures taken, which entails lying on a table with a rotating gamma scintillation camera moving around you.
After the resting pictures, you usually wait awhile to let the tracer flush out of your system, then it's on to the treadmill, with ECG leads in place to monitor you. Mine was 13 or 15 minutes (can't remember exactly) and 1 minute before the end, they inject another round of radioactive tracer. You have a bit of a wait, again to let the tracer circulate and deposit in the muscle cells. There is now another round of pictures to get blood delivery to the stressed heart muscle, and that is it.
This site has a good description:
http://www.heartsite.com/html/isotope_stress.html
Different centers may have some differences in the procedure, but it's generally as I describe. Most of the time will be spent waiting for the tracer to circulate, and lying on the camera table (each series of pictures during my test took about 25 minutes for the camera to rotate it's full 180 degrees - actually two camera's opposite eachother on a rotating arm).