Yes, that can happen. The one time that I relied on an OPK I missed my chance. I Oed early and the OPK never picked it up.

It wasn't until my temp had been raised for almost 5 days that I realized I had already Oed. OPKs are designed to detect your LH surge, which happens before you O, not when you already are Oing. The problems with this are: Many women have more than 1 LH surge before the real O actually happens. A lot of women see the first OPK and think that's it. Also, some women have an LH surge that comes and goes in a matter of hours. If you are one of those women you have to use the OPK twice a day or more in order to catch it. Personally, that's too much $$$ and too much of a bother for me.

I have much better luck with BBT charting and it doesn't cost me a thing. I know that a lot of women have a good deal of success with OPKs, I'm just not one of them. Hopefully they will work better for you!