Yes, but it's better to do it in the morning because that's when there is the most concentration of HCG if you are pregnant. If you want to do it in the afternoon, try not drinking any fluids for a couple hours first. Good luck,
I took both of my Fact Plus tests in the evening b/c I couldn't function well enough in the morning to open the durn foil wrapper! I guess I was preg. enough it didn't matter. I am 9 weeks preg. now and I found out through a hpt 3 1/2 weeks ago. That is 5 1/2 weeks preg. at the time. I think if you are the line will show up no matter when. (Yes both of my lines were dark enough to tell I was preg.)
Smile, life doesn't last that long.