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    Old 06-05-2007, 08:43 AM   #1
    zandvoort
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    Question need help reading my western blot results

    Hi- Would love some assistance. I went to the doc yesterday and he said I did not have lyme- none of the bands showed up. I wanted to be sure he meant NONE of the bands showed up and not just none of the CDC bands. So I looked at my test results. It does look like I do not have one single band but I wanted to check with people on this board. If there is any doubt, I want to get an Igenex test.

    Here is what the results look like:

    Lyme EIA 1.13 LIV (Equivocal if 0-1.2)

    Lyme G West Ref
    Bands present: NONE
    (Insufficient bands for a positive result)
    Test information: Borrelia Burgdorferi Ab, IgG Western blot IgG positive: Any 5 of the following 10 bands: 18,23,28,30,39,41,45,58,66 or 93 kDa.
    Result: Negative

    Lyme M West RFX
    Bands Present: NONE
    (Insufficient bands for a positive result)
    Test information: Borrelia Burgdorferi Ab, IgG Western blot IgG positive: Any 2 of the following 3 bands 23,39 or 41 kDa.
    Result: Negative

    Thanks!

     
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    Old 06-05-2007, 12:21 PM   #2
    ticker
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    Re: need help reading my western blot results

    Hi zandvoort. Which lab did the Western Blot? It is important to be tested by a Lyme lab such as IgeneX. They use more sensitive testing and list more bands on the Western Blot.

    The most important thing you can do to rule out or confirm Lyme disease is see a Lyme knoweldgeable doctor.

     
    Old 06-08-2007, 05:49 AM   #3
    zandvoort
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    Re: need help reading my western blot results

    Hi Ticker- the lab was ARUP. Have you heard of them? I've gone to their website to try and get some help with the reading but nothing there. I think it so odd to have NO bands present. I've had chicken pox- you'd at least think that would show up!

     
    Old 06-08-2007, 06:12 AM   #4
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    Re: need help reading my western blot results

    Hi zandvoort. I have never heard of this lab. I would get tested at IgeneX if I were you.

     
    Old 06-08-2007, 09:26 AM   #5
    Bill S
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    Re: need help reading my western blot results

    Quote:
    Originally Posted by zandvoort View Post
    Hi- Would love some assistance. I went to the doc yesterday and he said I did not have lyme- none of the bands showed up. I wanted to be sure he meant NONE of the bands showed up and not just none of the CDC bands. So I looked at my test results. It does look like I do not have one single band but I wanted to check with people on this board. If there is any doubt, I want to get an Igenex test.

    Here is what the results look like:

    Lyme EIA 1.13 LIV (Equivocal if 0-1.2)

    Lyme G West Ref
    Bands present: NONE
    (Insufficient bands for a positive result)
    Test information: Borrelia Burgdorferi Ab, IgG Western blot IgG positive: Any 5 of the following 10 bands: 18,23,28,30,39,41,45,58,66 or 93 kDa.
    Result: Negative

    Lyme M West RFX
    Bands Present: NONE
    (Insufficient bands for a positive result)
    Test information: Borrelia Burgdorferi Ab, IgG Western blot IgG positive: Any 2 of the following 3 bands 23,39 or 41 kDa.
    Result: Negative

    Thanks!
    Looks like this lab is using the old CDC-criteria of 5 bands, despite the CDC saying their criteria was to be used ONLY for their tracking purposes, not using it for diagnosis. I think Igenex uses 3 bands out of 10...and the Elisa is equivacable (ie, maybe positive). The Elisa isn't all that accurate anyways.

    Yep, I'd get re-tested by a reputable lab.

    Last edited by Bill S; 06-08-2007 at 09:28 AM.

     
    Old 06-08-2007, 10:15 AM   #6
    zandvoort
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    Re: need help reading my western blot results

    thanks for the info. I sent a fax this morning to a Dr Sam Donta, an LLMD in Boston, asking for a consultation.

     
    Old 06-08-2007, 11:28 AM   #7
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    Re: need help reading my western blot results

    Hi zandvoort, I do not recommend this doctor. Dr. Steven Phillips in Wilton, CT comes highly recommended.

     
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