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  • IGenex Results - Need Help Understanding

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    Old 03-09-2009, 08:13 AM   #1
    wdiguy
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    IGenex Results - Need Help Understanding

    Test Name: IFA B Burgdorferi G/M/A Result: <1:40 TITER

    Ranges:
    <1:40 Negative
    1:40 Inderterminate
    = OR >1:80 Positive





    Lyme IgM WESTERN BLOT

    IGenex Interpretation is based on internal validation stugies. By IGenex criteria, IgM WB is considered positive if two or more of the double starred bands are present. The Igm WB is considered negative if less than 2 starred bands are present. A positive suggests exposure to B burgdorferi. By CDC/NYS criteria, Igm WB is negative if less than 2 bands are present.
    LIMITATION: Positive result for 31 and/or 34kda may be present after vaccination in uninfected persons. Infection with HSV, EBV, HCV and/or syphillis (RPR+) may give false (+) results. In a sample set of 165 well characterized specimens by IGenex criteria; and 99% and 58% by CDC/NYS criteria respectively.
    ****PRESENCE OF ONLY ONE DOUBLE STARRED BAND OR INDETERMINATE DOUBLE STARRED BANDS IN A NEGATIVE REPORT MAY INDICATE CLINICAL SIGNIFICANCE**** THEREFORE, WE RECOMMEND TESTING WITH ANOTHER METHOD AND/OR RESTEST IN 4-6WEEKS.
    BAND INTENSITY: Negative (-) : No visible band present. Indeterminate (IND) : Band present with intensity < Weak (1+) Positive Control. Positive (1+ to 4+) : Band present at an intensity >/= Weak (1+) Positive Control.


    IGM Result NEGATIVE
    CDC/NYS Result NEGATIVE

    18 kda. -
    22 kda. -
    **23-25 kda. -
    28 kda. -
    30 kda. -
    **31 kda. IND
    **34 kda. -
    **39 kda. -
    **41 kda. IND
    45 kda. -
    58 kda. -
    66 kda. -
    73 kda. -
    **83-93 kda. -




    LYME IgG WESTERN BLOT

    IGenex interpretation is based on internal validation studies. By IGenex criteria, IgG WB is reported positive if two or more of the double starred bands below are present. IgG WB is negative is lees than 2 starred bands are present.
    A positive result suggests exposure to B burgdorferi.
    By CDC/NYS criteria, IgG WB is reported positive if 5 of the following bands are present: 18,23-25,28,30,39,41,45,58,66,83-93kda. The IgG WB is nagtive if less than 5 bands are present.
    LIMITATION: Positive result for 31 and/or 34kda may be present after vaccination in uninfected persons. In a sample set of 165 well characterized specimens with 36% positivity rate, the assay specificty and sensitivity was 96% & 63% by IGenex criteria; 100% and 38% by CDC/NYS criteria respectively.
    ****PRESENCE OF ONLY ONE DOUBLE STARRED BAND OR INDETERMINATE DOUBLE STARRED BANDS IN A NEGATIVE REPORT MAY INDICATE CLINICAL SIGNIFICANCE**** THEREFORE, WE RECOMMEND TESTING WITH ANOTHER METHOD AND/OR RESTEST IN 4-6WEEKS.
    BAND INTENSITY: Negative (-) : No visible band present. Indeterminate (IND) : Band present with intensity < Weak (1+) Positive Control. Positive (1+ to 4+) : Band present at an intensity >/= Weak (1+) Positive Control.

    Igenex - IGG - Result Negative
    CDC/NYS- Result Negative

    18 kda -
    22 kda -
    *23-25 kda -
    28 kda -
    30 kda -
    **31 kda -
    **34 kda -
    **39 kda -
    **41 kda +
    45 kda -
    58 kda -
    66 kda -
    73 kda -
    **83-93 kda -


    Whole Blood
    Genomic or Plasmid B burg NEGATIVE
    Plasmid - B burgdorferi NEGATIVE

    Serum
    Genomic or Plasmid B burg NEGATIVE
    Plasmid - B burgdorferi POSITIVE -Confirmed by Southern Dot Blot


    Any help would be greatly appreciated. Thanks.

     
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    Old 03-10-2009, 02:20 PM   #2
    wdiguy
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    Re: IGenex Results - Need Help Understanding

    Well..... I spoke to the doc at igenex labs. He said since that serum is positive you cant get a more specific than that. Very significant. He would go get treated if he was I.

     
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